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murine pancreatic α cell line αtc1  (ATCC)


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    Structured Review

    ATCC murine pancreatic α cell line αtc1
    Murine Pancreatic α Cell Line αtc1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1tc1+6+cells/alpha+TC1+clone+6/pm42219277-48-1-9
    Average 95 stars, based on 119 article reviews
    murine pancreatic α cell line αtc1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Mesenchymal stromal cells ameliorate mitochondrial dysfunction in α cells and hyperglucagonemia in type 2 diabetes via SIRT1/FoxO3a signaling.
    Article Snippet: .. Human embryo lung fibroblasts (HELFs) were obtained from the China Cell Culture Center and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium (Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C and in a 5% CO2 incubator. αTC1-6 cells were obtained from the American Type Culture Collection (ATCC Number: CRL-2934) and cultured in DMEM/high-glucose medium (Gibco) supplemented with 10% FBS and antibiotics. ..

    Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion
    Article Snippet: Experiments were conducted according to previously described methods , using a QBT assay (Molecular Devices). .. Briefly, αTC1-6 cells (American Type Culture Collection [ATCC]), originally derived from mouse pancreatic α cells , were seeded in 96-well plates and cultured to confluence over 24 hours. .. Wells were washed with serum-free, phenol-free media for 2 hours at 37° C. Lipofermata (5-bromo-5′-phenylspiro[3 H -1,3,4-thiadiazole-2,3′-indoline]-2-1) (MedChemExpress) was robotically incubated for the final hour.

    Article Title: Hyaluronan induces ERK activation with minimal transcriptomic changes in pancreatic α-cells
    Article Snippet: .. The αTC1-6 cells (ATCC, Manassas, VA, USA) were cultured in low-glucose Dulbecco's Modified Eagle's Medium (DMEM) (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% bovine serum albumin, penicillin (100 U/mL), and streptomycin (0.1 mg/mL) under 5% CO 2 at 37 °C. .. LMW hyaluronan (GLR001, 32 kDa, R&D Systems, Minneapolis, MN, USA) was dissolved in phosphate-buffered saline and applied at indicated concentrations.

    Article Title: Fatty acid transport protein-2 inhibition enhances glucose tolerance through α-cell-mediated GLP-1 secretion
    Article Snippet: Experiments were conducted according to previously described 353 methods (20), using a QBT assay (Molecular Devices). .. Briefly, αTC1-6 cells (ATCC), which were 354 originally derived from mouse pancreatic α-cells (64), were seeded in 96-well plates, and 355 cultured to confluence over 24 h. Wells were washed with serum-free, phenol-free media for 2 356 h at 37o C; Lipofermata (5-bromo-5ʹ-phenylspiro[ 357 3H-1,3,4-thiadiazole-2,3ʹ-indoline]-2-one) (MedChemExpress) was robotically incubated for the 358 final hour. .. BODIPY-conjugated C18 fatty acids (Molecular Devices QBT assay, 2.5 μM complexed 359 with 0.2 % fatty acid-free albumin carrier + FATP2 inhibitors in QBT loading buffer that contains a 360 proprietary external quenching dye) were robotically added at time = 0.

    Article Title: Mesenchymal stromal cells ameliorate mitochondrial dysfunction in α cells and hyperglucagonemia in type 2 diabetes via SIRT1/FoxO3a signaling
    Article Snippet: .. Human embryo lung fibroblasts (HELFs) were obtained from the China Cell Culture Center and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium (Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C and in a 5% CO 2 incubator. αTC1-6 cells were obtained from the American Type Culture Collection (ATCC Number: CRL-2934) and cultured in DMEM/high-glucose medium (Gibco) supplemented with 10% FBS and antibiotics. ..

    Article Title: Hyaluronan induces ERK activation with minimal transcriptomic changes in pancreatic α-cells.
    Article Snippet: .. The αTC1-6 cells (ATCC, Manassas, VA, USA) were cultured in lowglucose Dulbecco's Modified Eagle's Medium (DMEM) (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% bovine serum albumin, penicillin (100 U/mL), and streptomycin (0.1 mg/mL) under 5% CO2 at 37 ◦C. .. LMW hyaluronan (GLR001, 32 kDa, R&D Systems, Minneapolis, MN, USA) was dissolved in phosphate-buffered saline and applied at indicated concentrations.

    Modification:

    Article Title: Mesenchymal stromal cells ameliorate mitochondrial dysfunction in α cells and hyperglucagonemia in type 2 diabetes via SIRT1/FoxO3a signaling.
    Article Snippet: .. Human embryo lung fibroblasts (HELFs) were obtained from the China Cell Culture Center and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium (Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C and in a 5% CO2 incubator. αTC1-6 cells were obtained from the American Type Culture Collection (ATCC Number: CRL-2934) and cultured in DMEM/high-glucose medium (Gibco) supplemented with 10% FBS and antibiotics. ..

    Article Title: Hyaluronan induces ERK activation with minimal transcriptomic changes in pancreatic α-cells
    Article Snippet: .. The αTC1-6 cells (ATCC, Manassas, VA, USA) were cultured in low-glucose Dulbecco's Modified Eagle's Medium (DMEM) (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% bovine serum albumin, penicillin (100 U/mL), and streptomycin (0.1 mg/mL) under 5% CO 2 at 37 °C. .. LMW hyaluronan (GLR001, 32 kDa, R&D Systems, Minneapolis, MN, USA) was dissolved in phosphate-buffered saline and applied at indicated concentrations.

    Article Title: Mesenchymal stromal cells ameliorate mitochondrial dysfunction in α cells and hyperglucagonemia in type 2 diabetes via SIRT1/FoxO3a signaling
    Article Snippet: .. Human embryo lung fibroblasts (HELFs) were obtained from the China Cell Culture Center and cultured in Dulbecco’s modified Eagle’s medium (DMEM)/high-glucose medium (Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C and in a 5% CO 2 incubator. αTC1-6 cells were obtained from the American Type Culture Collection (ATCC Number: CRL-2934) and cultured in DMEM/high-glucose medium (Gibco) supplemented with 10% FBS and antibiotics. ..

    Article Title: Hyaluronan induces ERK activation with minimal transcriptomic changes in pancreatic α-cells.
    Article Snippet: .. The αTC1-6 cells (ATCC, Manassas, VA, USA) were cultured in lowglucose Dulbecco's Modified Eagle's Medium (DMEM) (Nacalai Tesque Inc., Kyoto, Japan) supplemented with 10% fetal bovine serum, 15 mM HEPES, 0.1 mM non-essential amino acids, 0.02% bovine serum albumin, penicillin (100 U/mL), and streptomycin (0.1 mg/mL) under 5% CO2 at 37 ◦C. .. LMW hyaluronan (GLR001, 32 kDa, R&D Systems, Minneapolis, MN, USA) was dissolved in phosphate-buffered saline and applied at indicated concentrations.

    Derivative Assay:

    Article Title: Fatty acid transport protein 2 inhibition enhances glucose tolerance through α cell–mediated GLP-1 secretion
    Article Snippet: Experiments were conducted according to previously described methods , using a QBT assay (Molecular Devices). .. Briefly, αTC1-6 cells (American Type Culture Collection [ATCC]), originally derived from mouse pancreatic α cells , were seeded in 96-well plates and cultured to confluence over 24 hours. .. Wells were washed with serum-free, phenol-free media for 2 hours at 37° C. Lipofermata (5-bromo-5′-phenylspiro[3 H -1,3,4-thiadiazole-2,3′-indoline]-2-1) (MedChemExpress) was robotically incubated for the final hour.

    Article Title: Fatty acid transport protein-2 inhibition enhances glucose tolerance through α-cell-mediated GLP-1 secretion
    Article Snippet: Experiments were conducted according to previously described 353 methods (20), using a QBT assay (Molecular Devices). .. Briefly, αTC1-6 cells (ATCC), which were 354 originally derived from mouse pancreatic α-cells (64), were seeded in 96-well plates, and 355 cultured to confluence over 24 h. Wells were washed with serum-free, phenol-free media for 2 356 h at 37o C; Lipofermata (5-bromo-5ʹ-phenylspiro[ 357 3H-1,3,4-thiadiazole-2,3ʹ-indoline]-2-one) (MedChemExpress) was robotically incubated for the 358 final hour. .. BODIPY-conjugated C18 fatty acids (Molecular Devices QBT assay, 2.5 μM complexed 359 with 0.2 % fatty acid-free albumin carrier + FATP2 inhibitors in QBT loading buffer that contains a 360 proprietary external quenching dye) were robotically added at time = 0.

    Incubation:

    Article Title: Fatty acid transport protein-2 inhibition enhances glucose tolerance through α-cell-mediated GLP-1 secretion
    Article Snippet: Experiments were conducted according to previously described 353 methods (20), using a QBT assay (Molecular Devices). .. Briefly, αTC1-6 cells (ATCC), which were 354 originally derived from mouse pancreatic α-cells (64), were seeded in 96-well plates, and 355 cultured to confluence over 24 h. Wells were washed with serum-free, phenol-free media for 2 356 h at 37o C; Lipofermata (5-bromo-5ʹ-phenylspiro[ 357 3H-1,3,4-thiadiazole-2,3ʹ-indoline]-2-one) (MedChemExpress) was robotically incubated for the 358 final hour. .. BODIPY-conjugated C18 fatty acids (Molecular Devices QBT assay, 2.5 μM complexed 359 with 0.2 % fatty acid-free albumin carrier + FATP2 inhibitors in QBT loading buffer that contains a 360 proprietary external quenching dye) were robotically added at time = 0.



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